Generation of hypoxia-sensing chimeric antigen receptor T cells.

ABSTRACT: Exploiting hypoxia in solid malignancies to restrict expression of chimeric antigen receptors (CARs) on engineered T cells to the tumor microenvironment overcomes the risk of on-target off-tumor toxicity and minimizes tonic signaling, which promotes CAR T cell exhaustion. This protocol summarizes the synthetic biology underlying the development of a stringent oxygen-sensitive CAR for in vitro and in vivo preclinical characterization. For complete details on the use and execution of this protocol, please refer to Kosti et al. (2021).

SUBMITTER: Kosti P 

PROVIDER: S-EPMC8353489 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature