Kruppel-like factor 5 regulates wound repair and the innate immune response in human airway epithelial cells.

ABSTRACT: A complex network of transcription factors regulates genes involved in establishing and maintaining key biological properties of the human airway epithelium. However, detailed knowledge of the contributing factors is incomplete. Here we characterize the role of Krüppel-like factor 5 (KLF5), in controlling essential pathways of epithelial cell identity and function in the human lung. RNA-seq following siRNA-mediated depletion of KLF5 in the Calu-3 lung epithelial cell line identified significant enrichment of genes encoding chemokines and cytokines involved in the proinflammatory response and also components of the junctional complexes mediating cell adhesion. To determine direct gene targets of KLF5, we defined the cistrome of KLF5 using ChIP-seq in both Calu-3 and 16HBE14o^- lun

SUBMITTER: Paranjapye A 

PROVIDER: S-EPMC8353497 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature