Differential gene regulation in DAPT-treated Hydra reveals candidate direct Notch signalling targets.

ABSTRACT: In Hydra, Notch inhibition causes defects in head patterning and prevents differentiation of proliferating nematocyte progenitor cells into mature nematocytes. To understand the molecular mechanisms by which the Notch pathway regulates these processes, we performed RNA-seq and identified genes that are differentially regulated in response to 48 h of treating the animals with the Notch inhibitor DAPT. To identify candidate direct regulators of Notch signalling, we profiled gene expression changes that occur during subsequent restoration of Notch activity and performed promoter analyses to identify RBPJ transcription factor-binding sites in the regulatory regions of Notch-responsive genes. Interrogating the available single-cell sequencing data set revealed the gene expression patterns of No

SUBMITTER: Moneer J 

PROVIDER: S-EPMC8353520 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature