Principles of nucleosome organization revealed by single-cell micrococcal nuclease sequencing.

ABSTRACT: Nucleosome positioning is critical to chromatin accessibility and is associated with gene expression programs in cells^1-3. Previous nucleosome mapping methods assemble profiles from cell populations and reveal a cell-averaged pattern: nucleosomes are positioned and form a phased array that surrounds the transcription start sites of active genes^3-6 and DNase I hypersensitive sites⁷. However, even in a homogenous population of cells, cells exhibit heterogeneity in expression in response to active signalling^8,9 that may be related to heterogeneity in chromatin accessibility^10-12. Here we report a technique, termed single-cell micrococcal nuclease sequencing (scMNase-seq), that can be used to simultaneously measure genome-wide nucleosome p

SUBMITTER: Lai B 

PROVIDER: S-EPMC8353605 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature