Microfluidic chip grafted with integrin tension sensors for evaluating the effects of flowing shear stress and ROCK inhibitor on platelets.

ABSTRACT: Integrins are key players in platelet adhesion and aggregation. Integrin molecular tensions, the forces transmitted by integrin molecules, are regulated by both mechanical and biochemical cues, and the outside-in and inside-out signaling has been extensively studied. While the mechanical properties of platelets at static status have been studied by atomic force microscopy, traction force microscopy and tension sensors, the biomechanical properties of flowing platelets remain elusive. Herein, we report microfluidic chips grafted with integrin tension sensors for microfluidic-force mapping in platelets. Specifically, the process of integrin α_IIbβ₃ mediating tension transmission and platelet adhesion under low flow rates has been obtained, and the process of platelet clu

SUBMITTER: Mao S 

PROVIDER: S-EPMC8353964 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature