Transcriptome Analysis of CCR9+ T Helper Cells From Primary Sjogren's Syndrome Patients Identifies CCL5 as a Novel Effector Molecule.

ABSTRACT:

Introduction

CCR9+ Tfh-like pathogenic T helper (Th) cells are elevated in patients with primary Sjögren's syndrome (pSS) and indicated to play a role in pSS immunopathology. Here we delineate the CCR9+ Th cell-specific transcriptome to study the molecular dysregulation of these cells in pSS patients.

Methods

CCR9+, CXCR5+ and CCR9-CXCR5- Th cells from blood of 7 healthy controls (HC) and 7 pSS patients were FACS sorted and RNA sequencing was performed. Computational analysis was used to identify differentially expressed genes (DEGs), coherent gene expression networks and differentially regulated pathways. Target genes were replicated in additional cohorts.

Results

5131 genes were differentially expressed between CCR9+ and CXCR5+ Th cells; 6493 and 4783 between CCR9+

SUBMITTER: Hinrichs AC 

PROVIDER: S-EPMC8354142 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature