Dataset Information
Anti-depressant effects of ethanol extract from Cannabis sativa (hemp) seed in chlorpromazine-induced Drosophila melanogaster depression model.
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ABSTRACT: Context
Depression is a severe mental illness caused by a deficiency of dopamine and serotonin. Cannabis sativa L. (Cannabaceae) has long been used to treat pain, nausea, and depression.Objective
This study investigates the anti-depressant effects of C. sativa (hemp) seed ethanol extract (HE) in chlorpromazine (CPZ)-induced Drosophila melanogaster depression model.Materials and methods
The normal group was untreated, and the control group was treated with CPZ (0.1% of media) for 7 days. The experimental groups were treated with a single HE treatment (0.5, 1.0, and 1.5% of media) and a mixture of 0.1% CPZ and HE for 7 days. The locomotor activity, behavioural patterns, depression-related gene expression, and neurotransmitters level of flies were investigated.Results
The behavioural patterns of individual flies were significantly reduced with 0.1% CPZ treatment. In contrast, combination treatment of 1.5% HE and 0.1% CPZ significantly increased subjective daytime activity (p < 0.001) and behavioural factors (p < 0.001). These results correlate with increased transcript levels of dopamine (p < 0.001) and serotonin (p < 0.05) receptors and concentration of dopamine (p < 0.05), levodopa (p < 0.001), 5-HTP (p < 0.05), and serotonin (p < 0.001) compared to those in the control group.Discussion and conclusions
Collectively, HE administration alleviates depression-like symptoms by modulating the circadian rhythm-related behaviours, transcript levels of neurotransmitter receptors, and neurotransmitter levels in the CPZ-induced Drosophila model. However, additional research is needed to investigate the role of HE administration in behavioural patterns, reduction of the neurotransmitter, and signalling pathways of depression in a vertebrate model system.
SUBMITTER: Ahn Y
PROVIDER: S-EPMC8354181 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
Anti-depressant effects of ethanol extract from <i>Cannabis sativa</i> (hemp) seed in chlorpromazine-induced <i>Drosophila melanogaster</i> depression model.
Pharmaceutical biology 20211201 1
<h4>Context</h4>Depression is a severe mental illness caused by a deficiency of dopamine and serotonin. <i>Cannabis sativa</i> L. (Cannabaceae) has long been used to treat pain, nausea, and depression.<h4>Objective</h4>This study investigates the anti-depressant effects of <i>C. sativa</i> (hemp) seed ethanol extract (HE) in chlorpromazine (CPZ)-induced <i>Drosophila melanogaster</i> depression model.<h4>Materials and methods</h4>The normal group was untreated, and the control group was treated ...[more]
