Downregulation of miR-142a Contributes to the Enhanced Anti-Apoptotic Ability of Murine Chronic Myelogenous Leukemia Cells.

ABSTRACT:

Background

Leukemic stem cell (LSC) is thought to be responsible for chronic myelogenous leukemia (CML) initiation and relapse. However, the inherent regulation of LSCs remains largely obscure. Herein, we integratedly analyzed miRNA and gene expression alterations in bone marrow (BM) Lin^-Sca1⁺c-Kit⁺ cells (LSKs) of a tet-off inducible CML mouse model, Scl/tTA-BCR/ABL (BA).

Methods

Scl/tTA and TRE-BA transgenic mice were crossed in the presence of doxycycline to get double transgenic mice. Both miRNA and mRNA expression profiles were generated from BM LSKs at 0 and 3 weeks after doxycycline withdrawal. The target genes of differentially expressed miRNAs were predicted, followed by the miRNA-mRNA network construction. In vitro and in

SUBMITTER: Chen Z 

PROVIDER: S-EPMC8354203 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature