Multidimensional study of the oral microbiome, metabolite, and immunologic environment in peanut allergy.

ABSTRACT:

Background

The oral mucosa is the initial interface between food antigens, microbiota, and mucosal immunity, yet, little is known about oral host-environment dynamics in food allergy.

Objective

Our aim was to determine oral microbial, metabolic, and immunologic profiles associated with peanut allergy.

Methods

We recruited 105 subjects (56 with peanut allergy and 49 healthy subjects) for salivary microbiome profiling using 16S ribosomal RNA sequencing, short-chain fatty acid (SCFA) metabolite assays using liquid chromatography/mass spectrometry, and measurement of oral secreted cytokines using multiplex assays. Analyses within and across data types were performed.

Results

The oral microbiome of individuals with peanut allergy was characterized by reduced species

SUBMITTER: Ho HE 

PROVIDER: S-EPMC8355025 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature