Compressed sensing for highly efficient imaging transcriptomics.

ABSTRACT: Recent methods for spatial imaging of tissue samples can identify up to ~100 individual proteins^1-3 or RNAs^4-10 at single-cell resolution. However, the number of proteins or genes that can be studied in these approaches is limited by long imaging times. Here we introduce Composite In Situ Imaging (CISI), a method that leverages structure in gene expression across both cells and tissues to limit the number of imaging cycles needed to obtain spatially resolved gene expression maps. CISI defines gene modules that can be detected using composite measurements from imaging probes for subsets of genes. The data are then decompressed to recover expression values for individual genes. CISI further reduces imaging time by not relying on spot-level resolution, enabling lower mag

SUBMITTER: Cleary B 

PROVIDER: S-EPMC8355028 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature