Ontology highlight
ABSTRACT:
SUBMITTER: Schimmel J
PROVIDER: S-EPMC8355091 | biostudies-literature | 2021 Aug
REPOSITORIES: biostudies-literature
Nature communications 20210810 1
Small tandem duplications of DNA occur frequently in the human genome and are implicated in the aetiology of certain human cancers. Recent studies have suggested that DNA double-strand breaks are causal to this mutational class, but the underlying mechanism remains elusive. Here, we identify a crucial role for DNA polymerase α (Pol α)-primase in tandem duplication formation at breaks having complementary 3' ssDNA protrusions. By including so-called primase deserts in CRISPR/Cas9-induced DNA brea ...[more]