Development of a defined medium for Corynebacterium glutamicum using urea as nitrogen source.

ABSTRACT: Corynebacterium glutamicum has been widely used for bulk and fine chemicals fermentation these years. In this study, we developed a defined medium for this bacteria based on the widely used CGXII minimal medium. We evaluated the effects of different components in CGXII on cell growth of C. glutamicum ATCC 13032 and improved the medium through single-factor experiment and central composite design (CCD). Urea, K₂HPO₄ and MgSO₄ were found to be significant factors. 7 out of the total 15 components were modified. (NH₄)₂SO₄, KH₂PO₄, and protocatechuic acid were eliminated. Amounts of urea and MgSO₄ were increased, and concentrations of biotin and glucose were reduced. The resulting R

SUBMITTER: Yang P 

PROVIDER: S-EPMC8355296 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature