The Metabolic Landscape of Thymic T Cell Development <i>In Vivo</i> and <i>In Vitro</i>.

ABSTRACT: Although metabolic pathways have been shown to control differentiation and activation in peripheral T cells, metabolic studies on thymic T cell development are still lacking, especially in human tissue. In this study, we use transcriptomics and extracellular flux analyses to investigate the metabolic profiles of primary thymic and in vitro-derived mouse and human thymocytes. Core metabolic pathways, specifically glycolysis and oxidative phosphorylation, undergo dramatic changes between the double-negative (DN), double-positive (DP), and mature single-positive (SP) stages in murine and human thymus. Remarkably, despite the absence of the complex multicellular thymic microenvironment, in vitro murine and human T cell development recapitulated the coordinated decrease in glycoly

SUBMITTER: Sun V 

PROVIDER: S-EPMC8355594 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature