Astragaloside IV alleviates heart failure by regulating SUMO-specific protease 1.

ABSTRACT: The present study investigated whether the protective effect and mechanism of astragaloside IV (AS-IV) on heart failure (HF) involves small ubiquitin-like modifier (SUMO)-specific protease 1 (Senp1). Mouse HF was established by aortic constriction, inducing pressure overload. The model was confirmed by echocardiography 6 weeks after surgery. Mice were randomly divided into control, HF, HF+AS-IV, and AS-IV groups. Ventricular function was examined by echocardiography. Morphological changes of myocardial tissues were examined by H&E staining. The protein levels of the apoptosis-related proteins, cleaved caspase-3, caspase-3, Bcl2, Bax, and SUMO-Senp1 were determined by Western blotting. H₂O₂ in isolated mitochondria and cells was determined by Amplex Red. A reactive oxy

SUBMITTER: Liu J 

PROVIDER: S-EPMC8355636 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature