Molecular neural crest cell markers enable discrimination of organophosphates in the murine cardiac embryonic stem cell test.

ABSTRACT: The cardiac embryonic stem cell test (ESTc) originally used the differentiation of beating cardiomyocytes for embryotoxicity screenings of compounds. However, the ESTc consists of a heterogeneous cell population, including neural crest (NC) cells, which are important contributors to heart development in vivo. Molecular markers for NC cells were investigated to explore if this approach improved discrimination between structurally related chemicals, using the three organophosphates (OP): chlorpyrifos (CPF), malathion (MLT), and triphenyl phosphate (TPP). To decrease the test duration and to improve the objective quantification of the assay read-out, gene transcript biomarkers were measured on study day 4 instead of the traditional cardiomyocyte beating assessment at day 10. Gene expre

SUBMITTER: Mennen RH 

PROVIDER: S-EPMC8355823 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature