Hepatitis C reference viruses highlight potent antibody responses and diverse viral functional interactions with neutralising antibodies.

ABSTRACT:

Objective

Neutralising antibodies are key effectors of infection-induced and vaccine-induced immunity. Quantification of antibodies' breadth and potency is critical for understanding the mechanisms of protection and for prioritisation of vaccines. Here, we used a unique collection of human specimens and HCV strains to develop HCV reference viruses for quantification of neutralising antibodies, and to investigate viral functional diversity.

Design

We profiled neutralisation potency of polyclonal immunoglobulins from 104 patients infected with HCV genotype (GT) 1-6 across 13 HCV strains representing five viral GTs. Using metric multidimensional scaling, we plotted HCV neutralisation onto neutralisation maps. We employed K-means clustering to guide virus clustering and selectin

SUBMITTER: Bankwitz D 

PROVIDER: S-EPMC8355883 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature