Use of dual-electron probes reveals the role of ferritin as an iron depot in ex vivo erythropoiesis.

ABSTRACT: In the finely regulated process of mammalian erythropoiesis, the path of the labile iron pool into mitochondria for heme production is not well understood. Existing models for erythropoiesis do not include a central role for the ubiquitous iron storage protein ferritin; one model proposes that incoming endosomal Fe³⁺ bound to transferrin enters the cytoplasm through an ion transporter after reduction to Fe²⁺ and is taken up into mitochondria through mitoferrin-1 transporter. Here, we apply a dual three-dimensional imaging and spectroscopic technique, based on scanned electron probes, to measure Fe³⁺ in ex vivo human hematopoietic stem cells. After seven days in culture, we observe cells displaying a highly specialized architecture with anchored clust

SUBMITTER: Aronova MA 

PROVIDER: S-EPMC8355919 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature