Using single-nucleus RNA-sequencing to interrogate transcriptomic profiles of archived human pancreatic islets.

ABSTRACT:

Background

Human pancreatic islets are a central focus of research in metabolic studies. Transcriptomics is frequently used to interrogate alterations in cultured human islet cells using single-cell RNA-sequencing (scRNA-seq). We introduce single-nucleus RNA-sequencing (snRNA-seq) as an alternative approach for investigating transplanted human islets.

Methods

The Nuclei EZ protocol was used to obtain nuclear preparations from fresh and frozen human islet cells. Such preparations were first used to generate snRNA-seq datasets and compared to scRNA-seq output obtained from cells from the same donor. Finally, we employed snRNA-seq to obtain the transcriptomic profile of archived human islets engrafted in immunodeficient animals.

Results

We observed virtually complete con

SUBMITTER: Basile G 

PROVIDER: S-EPMC8356387 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature