Microglial inclusions and neurofilament light chain release follow neuronal α-synuclein lesions in long-term brain slice cultures.

ABSTRACT:

Background

Proteopathic brain lesions are a hallmark of many age-related neurodegenerative diseases including synucleinopathies and develop at least a decade before the onset of clinical symptoms. Thus, understanding of the initiation and propagation of such lesions is key for developing therapeutics to delay or halt disease progression.

Methods

Alpha-synuclein (αS) inclusions were induced in long-term murine and human slice cultures by seeded aggregation. An αS seed-recognizing human antibody was tested for blocking seeding and/or spreading of the αS lesions. Release of neurofilament light chain (NfL) into the culture medium was assessed.

Results

To study initial stages of α-synucleinopathies, we induced αS inclusions in murine hippocampal slice cultures by seeded ag

SUBMITTER: Barth M 

PROVIDER: S-EPMC8356412 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature