PPR-DYW Protein EMP17 Is Required for Mitochondrial RNA Editing, Complex III Biogenesis, and Seed Development in Maize.

ABSTRACT: The conversion of cytidines to uridines (C-to-U) at specific sites in mitochondrial and plastid transcripts is a post-transcriptional processing event that is important to the expression of organellar genes. Pentatricopeptide repeat (PPR) proteins are involved in this process. In this study, we report the function of a previously uncharacterized PPR-DYW protein, Empty pericarp17 (EMP17), in the C-to-U editing and kernel development in maize. EMP17 is targeted to mitochondria. The loss-function of EMP17 arrests maize kernel development, abolishes the editing at ccmF _C -799 and nad2-677 sites, and reduces the editing at ccmF _C -906 and -966 sites. The absence of editing causes amino acid residue changes in CcmF_C-267 (Ser to

SUBMITTER: Wang Y 

PROVIDER: S-EPMC8357149 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature