A low Smc flux avoids collisions and facilitates chromosome organization in Bacillus subtilis.

ABSTRACT: SMC complexes are widely conserved ATP-powered DNA-loop-extrusion motors indispensable for organizing and faithfully segregating chromosomes. How SMC complexes translocate along DNA for loop extrusion and what happens when two complexes meet on the same DNA molecule is largely unknown. Revealing the origins and the consequences of SMC encounters is crucial for understanding the folding process not only of bacterial, but also of eukaryotic chromosomes. Here, we uncover several factors that influence bacterial chromosome organization by modulating the probability of such clashes. These factors include the number, the strength, and the distribution of Smc loading sites, the residency time on the chromosome, the translocation rate, and the cellular abundance of Smc complexes. By studying vario

SUBMITTER: Anchimiuk A 

PROVIDER: S-EPMC8357415 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature