Decidualized endometrial stromal cells present with altered androgen response in PCOS.

ABSTRACT: Hyperandrogenic women with PCOS show disrupted decidualization (DE) and placentation. Dihydrotestosterone (DHT) is reported to enhance DE in non-PCOS endometrial stromal cells (eSC_Ctrl); however, this has not been assessed in PCOS cells (eSC_PCOS). Therefore, we studied the transcriptome profile of non-decidualized (non-DE) and DE eSCs from women with PCOS and Ctrl in response to short-term estradiol (E2) and/or progesterone (P4) exposure with/without (±) DHT. The non-DE eSCs were subjected to E2 ± DHT treatment, whereas the DE (0.5 mM 8-Br-cAMP, 96 h) eSCs were post-treated with E2 and P4 ± DHT, and RNA-sequenced. Validation was performed by immunofluorescence and immunohistochemistry. The results showed that, regardless of treatment, the PCOS and Ctrl samples cluster

SUBMITTER: Khatun M 

PROVIDER: S-EPMC8357821 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature