Discovery of Conserved and Novel MicroRNAs in Galdieria sulphuraria.

ABSTRACT:

Background

As a thermoacidophilic microalga, Galdieria sulphuraria has a unique biological function. MicroRNA (miRNA) plays an important regulating role in plant various stress responses.

Objective

In this study, we identified lots of conserved and novel miRNAs in G. sulphuraria (gsu-miRNAs), and predicted their putative targets for the first time.

Materials and methods

Conserved and novel gsu-miRNAs were predicted via deep sequencing on the Illumina HiSeq 4000 platform combined with bioinformatics analysis with a series of filtration criteria. Characterization of gsu-miRNAs and their targets were searched by different bioinformatics software. Some gsu-miRNAs were validated by Northern blot and RT-PCR analysis. MiRNA target gene function was predicted vi

SUBMITTER: Gao F 

PROVIDER: S-EPMC8358169 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature