B and T cell response to SARS-CoV-2 vaccination in health care professionals with and without previous COVID-19.

ABSTRACT:

Background

In recent months numerous health care professional acquired COVID-19 at the workplace resulting in significant shortages in medical and nursing staff. We investigated how prior COVID-19 affects SARS-CoV-2 vaccination and how such knowledge could facilitate frugal vaccination strategies.

Methods

In a cohort of 41 healthcare professionals with (n=14) and without (n=27) previous SARS-CoV-2 infection, we assessed the immune status before, during and after vaccination with BNT162b2. The humoral immune response was assessed by receptor binding domain ELISA and different SARS-CoV-2 neutralisation assays using wildtype and pseudo-typed viruses. T cell immunity against SARS-CoV-2 surface and nucleocapsid peptides were studied using interferon-γ release assays and intracell

SUBMITTER: Zollner A 

PROVIDER: S-EPMC8358275 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature