A Timed Off-Switch for Dynamic Control of Gene Expression in Corynebacterium Glutamicum.

ABSTRACT: Dynamic control of gene expression mainly relies on inducible systems, which require supplementation of (costly) inducer molecules. In contrast, synthetic regulatory circuits, which allow the timed shutdown of gene expression, are rarely available and therefore represent highly attractive tools for metabolic engineering. To achieve this, we utilized the VanR/P _vanABK ^* regulatory system of Corynebacterium glutamicum, which consists of the transcriptional repressor VanR and a modified promoter of the vanABK operon (P _vanABK ^*). VanR activity is modulated by one of the phenolic compounds ferulic acid, vanillin or vanillic acid, which are co-metabolized with d-glucose. Thus, gene expression in the presence of d-glucose is

SUBMITTER: Siebert D 

PROVIDER: S-EPMC8358305 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature