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Fluorescent in situ mRNA detection in the adult mouse cochlea.

ABSTRACT: Ossification and the delicateness of the cochlear duct make histologic assessments of the mature cochlea a challenging endeavor. Treatments to soften the bone facilitate sectioning and dissection of the cochlear duct but limit in situ mRNA detection in such specimens. Here, we provide a protocol for in situ mRNA detection using hybridization chain reaction in whole-mount preparations of the adult mouse cochlea. We show examples for multi-probe detection of different mRNAs and describe combination of this method with conventional immunohistochemistry.

SUBMITTER: Diaz GH

PROVIDER: S-EPMC8358468 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Fluorescent in situ mRNA detection in the adult mouse cochlea.

Diaz Giovanni H GH Heller Stefan S

STAR protocols 20210807 3

Ossification and the delicateness of the cochlear duct make histologic assessments of the mature cochlea a challenging endeavor. Treatments to soften the bone facilitate sectioning and dissection of the cochlear duct but limit in situ mRNA detection in such specimens. Here, we provide a protocol for in situ mRNA detection using hybridization chain reaction in whole-mount preparations of the adult mouse cochlea. We show examples for multi-probe detection of different mRNAs and descr ...[more]

PMID: 34401777

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