A First-In-Class, Humanized Antibody Targeting Alternatively Spliced Tissue Factor: Preclinical Evaluation in an Orthotopic Model of Pancreatic Ductal Adenocarcinoma.

ABSTRACT: In 2021, pancreatic ductal adenocarcinoma (PDAC) is the 3^rd leading cause of cancer deaths in the United States. This is largely due to a lack of symptoms and limited treatment options, which extend survival by only a few weeks. There is thus an urgent need to develop new therapies effective against PDAC. Previously, we have shown that the growth of PDAC cells is suppressed when they are co-implanted with RabMab1, a rabbit monoclonal antibody specific for human alternatively spliced tissue factor (asTF). Here, we report on humanization of RabMab1, evaluation of its binding characteristics, and assessment of its in vivo properties. hRabMab1 binds asTF with a K_D in the picomolar range; suppresses the migration of high-grade Pt45.P1 cells in Boyden chamber assays;

SUBMITTER: Lewis CS 

PROVIDER: S-EPMC8358774 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature