Agonism of the α₇-acetylcholine receptor/PI3K/Akt pathway promotes neuronal survival after subarachnoid hemorrhage in mice.

ABSTRACT: Subarachnoid hemorrhage (SAH) results in severe neuronal dysfunction and degeneration. Since the nicotinic acetylcholine α₇ receptors (α₇-AChR) are involved in neuronal function and survival, we investigated if stimulation of α₇-AChR would promote neuronal survival and improve behavioral outcome following SAH in mice. Male mice subjected to SAH were treated with either galantamine (α₇-AChR agonist) or vehicle. Neurobehavioral testing was performed 24 h after SAH, and mice were euthanized for analysis of neuronal cell death or a cell survival (PI3K/Akt) signaling pathway. Neuron cell cultures were subjected to hemoglobin toxicity to assess the direct effects of α₇-AChR agonism independent of other cells. Treatment with the α₇

SUBMITTER: Dienel A 

PROVIDER: S-EPMC8359014 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature