Isolation and identification of exosomes from feline plasma, urine and adipose-derived mesenchymal stem cells.

ABSTRACT:

Background

Exosomes, internal proteins, lipids, and nucleic acids coated by phospholipid bilayer membranes, are one type of small extracellular vesicles, which can mediate cell-cell communication. In recent years, exosomes have gained considerable scientific interest due to their widely applied prospect in the diagnosis and therapeutics of human and animal diseases. In this study, we describe for the first time a feasible method designed to isolate and characterize exosomes from feline plasma, urine and adipose-derived mesenchymal stem cells.

Results

Exosomes from feline plasma, urine and adipose-derived mesenchymal stem cells were successfully isolated by differential centrifugation. Quantification and sizing of exosomes were assessed by transmission electron microscopy, fl

SUBMITTER: Li D 

PROVIDER: S-EPMC8359027 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature