Silica-coated magnetic nanoparticles activate microglia and induce neurotoxic D-serine secretion.

ABSTRACT:

Background

Nanoparticles have been studied for brain imaging, diagnosis, and drug delivery owing to their versatile properties due to their small sizes. However, there are growing concerns that nanoparticles may exert toxic effects in the brain. In this study, we assessed direct nanotoxicity on microglia, the resident macrophages of the central nervous system, and indirect toxicity on neuronal cells exerted by silica-coated magnetic nanoparticles containing rhodamine B isothiocyanate dye [MNPs@SiO₂(RITC)].

Methods

We investigated MNPs@SiO₂(RITC)-induced biological changes in BV2 murine microglial cells via RNA-sequencing-based transcriptome analysis and gas chromatography-mass spectrometry-based intracellular and extracellular amino acid profiling. Morp

SUBMITTER: Shin TH 

PROVIDER: S-EPMC8359100 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature