Simple Field Storage of Fish Samples for Measurement of DNA Content by Flow Cytometry.

ABSTRACT: Flow cytometry is an effective and widely used tool for determination of ploidy in fish, but it is not always possible to access the fresh samples for analysis. We investigated the potential for extended storage of fish tissue with sterlet and tench as representative species of Chondrostei and Teleostei, using blood and fin of subadult/adult specimens and tail of larvae. Thirteen procedures for extending storage, selected for rapidity and simplicity in both field and laboratory conditions, were tested for each tissue sample. Flow cytometry was applied to fresh tissue immediately after sampling and to tissue subjected to experimental protocols, always along with species-specific standard, after 1, 5, and 10 days storage at 0-4°C or freezing at -80°C. The fluorochrome 4',6-diamidine-2'-pheny

SUBMITTER: Hubalek M 

PROVIDER: S-EPMC8359303 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature