A comparison of four commercially available RNA extraction kits for wastewater surveillance of SARS-CoV-2 in a college population.

ABSTRACT: Localized wastewater surveillance has allowed for public health officials to gain a broader understanding of SARS-CoV-2 viral prevalence in the community allowing public health officials time to prepare for impending outbreaks. Given variable levels of virus in the population through public health interventions, proper concentration and extraction of viral RNA is a key step in ensuring accurate detections. With many commercial RNA extraction kits and methodologies available, the performance of 4 different kits were evaluated for SARS-CoV-2 RNA detection in wastewater, specifically focusing on their applicability to lower population densities such as those at university campus dorms. Raw wastewater samples were collected at 4 sites on a college campus over a 24 hour period as a composite sa

SUBMITTER: O'Brien M 

PROVIDER: S-EPMC8359497 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature