Obese rats intervened with Rhizoma coptidis revealed differential gene expression and microbiota by serum metabolomics.

ABSTRACT:

Background

Integrating systems biology is an approach for investigating metabolic diseases in humans. However, few studies use this approach to investigate the mechanism by which Rhizoma coptidis (RC) reduces the effect of lipids and glucose on high-fat induced obesity in rats.

Methods

Twenty-four specific pathogen-free (SPF) male Sprague-Dawley rats (80 ± 10 g) were used in this study. Serum metabolomics were detected by ultra-high-performance liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry. Liver tissue and cecum feces were used for RNA-Seq technology and 16S rRNA gene sequencing, respectively.

Results

We identified nine potential biomarkers, which are differential metabolites in the Control, Model and RC groups, including linol

SUBMITTER: Ji Y 

PROVIDER: S-EPMC8359625 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature