Ontology highlight
ABSTRACT:
SUBMITTER: Saroay R
PROVIDER: S-EPMC8359946 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
Saroay Rakesh R Roiban Gheorghe-Doru GD Alkhalaf Lona M LM Challis Gregory L GL
Chembiochem : a European journal of chemical biology 20210507 13
Aromatic nitration reactions are a cornerstone of organic chemistry, but are challenging to scale due to corrosive reagents and elevated temperatures. The cytochrome P450 TxtE nitrates the indole 4-position of l-tryptophan at room temperature using NO, O<sub>2</sub> and NADPH, and has potential to be developed into a useful aromatic nitration biocatalyst. However, its narrow substrate scope (requiring both the α-amino acid and indole functionalities) have hindered this. Screening of an R59 mutan ...[more]