Histology-based profile of inflammatory mediators in experimentally induced pulpitis in a rat model: screening for possible biomarkers.

ABSTRACT:

Aim

To profile molecular changes in lipopolysaccharide (LPS)-induced experimental pulpitis in a rat model and explore the feasibility of a molecular-based diagnostic strategy for pulpitis.

Methodology

Seventy-three maxillary incisors of Sprague-Dawley rats were used to establish pulpitis models with LPS. Inflammatory grading was performed in four equal sections of the pulp divided from the injured site to the root apex. An antibody array was used to compare the expression of 67 molecules between control pulp and inflamed pulp 12 and 72 h after LPS application. The levels of differentially expressed molecules in the control and inflamed pulp (collected at 3, 6, 9, 12, 24 and 72 h after LPS treatment) were examined via ELISA, and correlations between inflammatory scores and mo

SUBMITTER: Li M 

PROVIDER: S-EPMC8360108 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature