Rapid whole-blood assay to detect SARS-CoV-2-specific memory T-cell immunity following a single dose of AstraZeneca ChAdOx1-S COVID-19 vaccine.

ABSTRACT:

Objectives

With the ongoing emergence of SARS-CoV-2 variants and potential to evade vaccine-induced neutralisation, understanding the magnitude and breadth of vaccine-induced T-cell immunity will be critical for the ongoing optimisation of vaccine approaches. Strategies that provide a rapid and easily translatable means of assessing virus-specific T-cell responses provide an opportunity to monitor the impact of vaccine rollouts in the community. In this study, we assessed whether our recently developed SARS-CoV-2 whole-blood assay could be used effectively to analyse T-cell responses following vaccination.

Methods

Following a median of 15 days after the first dose of the ChAdOx1-S (AstraZeneca^®) vaccine, peripheral blood was isolated from 58 participants. Blood wa

SUBMITTER: Lineburg KE 

PROVIDER: S-EPMC8360255 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature