Benzylmalonyl-CoA dehydrogenase, an enzyme involved in bacterial auxin degradation.

ABSTRACT: A novel acyl-CoA dehydrogenase involved in degradation of the auxin indoleacetate by Aromatoleum aromaticum was identified as a decarboxylating benzylmalonyl-CoA dehydrogenase (IaaF). It is encoded within the iaa operon coding for enzymes of indoleacetate catabolism. Using enzymatically produced benzylmalonyl-CoA, the reaction was characterized as simultaneous oxidation and decarboxylation of benzylmalonyl-CoA to cinnamoyl-CoA and CO₂. Oxygen served as electron acceptor and was reduced to H₂O₂, whereas electron transfer flavoprotein or artificial dyes serving as electron acceptors for other acyl-CoA dehydrogenases were not used. The enzyme is homotetrameric, contains an FAD cofactor and is enantiospecific in benzylmalonyl-CoA turnover. It shows high catalyt

SUBMITTER: Schuhle K 

PROVIDER: S-EPMC8360864 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature