Rhamnolipid Enhances the Nitrogen Fixation Activity of Azotobacter chroococcum by Influencing Lysine Succinylation.

ABSTRACT: The enhancement of nitrogen fixation activity of diazotrophs is essential for safe crop production. Lysine succinylation (K^Suc) is widely present in eukaryotes and prokaryotes and regulates various biological process. However, knowledge of the extent of K^Suc in nitrogen fixation of Azotobacter chroococcum is scarce. In this study, we found that 250 mg/l of rhamnolipid (RL) significantly increased the nitrogen fixation activity of A. chroococcum by 39%, as compared with the control. Real-time quantitative reverse transcription PCR (qRT-PCR) confirmed that RL could remarkably increase the transcript levels of nifA and nifHDK genes. In addition, a global K^Suc of A. chroococcum was profiled using a 4D label-free quantitative p

SUBMITTER: Li J 

PROVIDER: S-EPMC8360865 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature