Tailoring the evolution of BL21(DE3) uncovers a key role for RNA stability in gene expression toxicity.

ABSTRACT: Gene expression toxicity is an important biological phenomenon and a major bottleneck in biotechnology. Escherichia coli BL21(DE3) is the most popular choice for recombinant protein production, and various derivatives have been evolved or engineered to facilitate improved yield and tolerance to toxic genes. However, previous efforts to evolve BL21, such as the Walker strains C41 and C43, resulted only in decreased expression strength of the T7 system. This reveals little about the mechanisms at play and constitutes only marginal progress towards a generally higher producing cell factory. Here, we restrict the solution space for BL21(DE3) to evolve tolerance and isolate a mutant strain Evo21(DE3) with a truncation in the essential RNase E. This suggests that RNA stability plays a central ro

SUBMITTER: Heyde SAH 

PROVIDER: S-EPMC8361080 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature