Synthetic design of farnesyl-electrostatic peptides for development of a protein kinase A membrane translocation switch.

ABSTRACT: Molecular switches that respond to a biochemical stimulus in cells have proven utility as a foundation for developing molecular sensors and actuators that could be used to address important biological questions. Developing a molecular switch unfortunately remains difficult as it requires elaborate coordination of sensing and actuation mechanisms built into a single molecule. Here, we rationally designed a molecular switch that changes its subcellular localization in response to an intended stimulus such as an activator of protein kinase A (PKA). By arranging the sequence for Kemptide in tandem, we designed a farnesylated peptide whose localization can dramatically change upon phosphorylation by PKA. After testing a different valence number of Kemptide as well as modulating the linker seque

SUBMITTER: Kim AK 

PROVIDER: S-EPMC8361095 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature