Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins.
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ABSTRACT:
SUBMITTER: Hirschenberger M
PROVIDER: S-EPMC8361205 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins.
Hirschenberger Maximilian M Hayn Manuel M Laliberté Alexandre A Koepke Lennart L Kirchhoff Frank F Sparrer Konstantin Maria Johannes KMJ
STAR protocols 20210813 4
We present a protocol for analyzing the impact of SARS-CoV-2 proteins in interferon signaling using luciferase reporter assays. Here, the induction of defined promoters can be quantitatively assessed with high sensitivity and broad linear range. The results are similar to those obtained using qPCR to measure endogenous mRNA induction. The assay requires stringent normalization and confirmation of the results in more physiological settings. The protocol is adaptable for other viruses and other in ...[more]
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