A luminescence-based protocol for assessing fructose metabolism via quantification of ketohexokinase enzymatic activity in mouse or human hepatocytes.
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ABSTRACT:
SUBMITTER: Park SH
PROVIDER: S-EPMC8361265 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
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A luminescence-based protocol for assessing fructose metabolism via quantification of ketohexokinase enzymatic activity in mouse or human hepatocytes.
Park Se-Hyung SH Helsley Robert N RN Noetzli Leila L Tu Ho-Chou HC Wallenius Kristina K O'Mahony Gavin G Boucher Jeremie J Liu Jianming J Softic Samir S
STAR protocols 20210809 3
Ketohexokinase (KHK) catalyzes the first step of fructose metabolism. Inhibitors of KHK enzymatic activity are being evaluated in clinical trials for the treatment of non-alcoholic fatty liver disease (NAFLD) and diabetes. Here, we present a luminescence-based protocol to quantify KHK activity. The accuracy of this technique has been validated using knockdown and overexpression of KHK <i>in vivo</i> and <i>in vitro</i>. The specificity of the assay has been verified using 3-O-methyl-D-fructose, ...[more]
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2024-11-14 | GSE278322 | GEO
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