Pyocin efficacy in a murine model of Pseudomonas aeruginosa sepsis.

ABSTRACT:

Background

Bloodstream infections with antibiotic-resistant Pseudomonas aeruginosa are common and increasingly difficult to treat. Pyocins are naturally occurring protein antibiotics produced by P. aeruginosa that have potential for human use.

Objectives

To determine if pyocin treatment is effective in a murine model of sepsis with P. aeruginosa.

Methods

Recombinant pyocins S5 and AP41 were purified and tested for efficacy in a Galleria mellonella infection model and a murine model of P. aeruginosa sepsis.

Results

Both pyocins produced no adverse effects when injected alone into mice and showed good in vitro antipseudomonal activity. In an invertebrate model of sepsis using G. mellonella, both pyocins significantly prolonged survival from 1/10 (10%) survival in

SUBMITTER: Six A 

PROVIDER: S-EPMC8361349 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature