Effect of plasma processing and storage on microparticle abundance, nitric oxide scavenging, and vasoactivity.

ABSTRACT:

Background

We set out to define the impact of collection, processing, and storage on plasma product microparticle (MP) abundance, potential for nitric oxide (NO) scavenging, and vasoactivity.

Study design and methods

Three currently US licensed products were tested: liquid plasma (LP), fresh frozen plasma (FFP), and solvent detergent plasma (SDP), along with a product under development, spray-dried solvent detergent plasma (SD-SDP) with/without beads. Vasoactivity was assessed in vitro using rabbit aortic vascular rings; MP abundance was determined by flow cytometry; and NO scavenging capacity/rate was determined using a biochemical NO consumption assay. All samples were analyzed unprocessed and following centrifugation at two speeds (2,500× g to remove platelets, and 25,000

SUBMITTER: Rogers SC 

PROVIDER: S-EPMC8361847 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature