Low-affinity but high-avidity interactions may offer an explanation for IgE-mediated allergen cross-reactivity.

ABSTRACT:

Background

Allergy is a global disease with overall frequencies of >20%. Symptoms vary from irritating local itching to life-threatening systemic anaphylaxis. Even though allergies are allergen-specific, there is a wide range of cross-reactivities (eg apple and latex) that remain largely unexplained. Given the abilities of low-affinity IgG antibodies to inhibit mast cells activation, here we elucidate the minimal affinity of IgE antibodies to induce type I hypersensitivity.

Methods

Three mature (high-affinity) IgE antibodies recognizing three distinct epitopes on Fel d 1, the major cat allergen, were back-mutated to germline conformation, resulting in binding to Fel d 1 with low affinity. The ability of these IgE antibodies to activate mast cells in vitro and in vivo was tes

SUBMITTER: Chang X 

PROVIDER: S-EPMC8361967 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature