Metabolic Landscape of the Mouse Liver by Quantitative ³¹ P Nuclear Magnetic Resonance Analysis of the Phosphorome.

ABSTRACT:

Background and aims

The liver plays a central role in all metabolic processes in the body. However, precise characterization of liver metabolism is often obscured by its inherent complexity. Phosphorylated metabolites occupy a prominent position in all anabolic and catabolic pathways. Here, we develop a ³¹ P nuclear magnetic resonance (NMR)-based method to study the liver "phosphorome" through the simultaneous identification and quantification of multiple hydrophilic and hydrophobic phosphorylated metabolites.

Approach and results

We applied this technique to define the metabolic landscape in livers from a mouse model of the rare disease disorder congenital erythropoietic porphyria (CEP) as well as two well-known murine models of nonalcoholic steatohepatitis: one

SUBMITTER: Bernardo-Seisdedos G 

PROVIDER: S-EPMC8362057 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature