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Tailored Mobility in a Zeolite Imidazolate Framework (ZIF) Antibody Conjugate*.

ABSTRACT: Zeolitic imidazolate framework (ZIF) hybrid fluorescent nanoparticles and ZIF antibody conjugates have been synthesized, characterized, and employed in lateral-flow immunoassay (LFIA). The bright fluorescence of the conjugates and the possibility to tailor their mobility gives a huge potential for diagnostic assays. An enzyme-linked immunosorbent assay (ELISA) with horseradish peroxidase (HRP) as label, proved the integrity, stability, and dispersibility of the antibody conjugates, LC-MS/MS provided evidence that a covalent link was established between these metal-organic frameworks and lysine residues in IgG antibodies.

SUBMITTER: Chapartegui-Arias A

PROVIDER: S-EPMC8362128 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Tailored Mobility in a Zeolite Imidazolate Framework (ZIF) Antibody Conjugate*.

Chapartegui-Arias Ander A Raysyan Anna A Belenguer Ana M AM Jaeger Carsten C Tchipilov Teodor T Prinz Carsten C Abad Carlos C Beyer Sebastian S Schneider Rudolf J RJ Emmerling Franziska F

Chemistry (Weinheim an der Bergstrasse, Germany) 20210521 36

Zeolitic imidazolate framework (ZIF) hybrid fluorescent nanoparticles and ZIF antibody conjugates have been synthesized, characterized, and employed in lateral-flow immunoassay (LFIA). The bright fluorescence of the conjugates and the possibility to tailor their mobility gives a huge potential for diagnostic assays. An enzyme-linked immunosorbent assay (ELISA) with horseradish peroxidase (HRP) as label, proved the integrity, stability, and dispersibility of the antibody conjugates, LC-MS/MS prov ...[more]

PMID: 33786901

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