In Vivo Imaging of Newt Lens Regeneration: Novel Insights Into the Regeneration Process.

ABSTRACT:

Purpose

To establish optical coherence tomography (OCT) as an in vivo imaging modality for investigating the process of newt lens regeneration.

Methods

Spectral-domain OCT was employed for in vivo imaging of the newt lens regeneration process. A total of 37 newts were lentectomized and followed by OCT imaging over the course of 60 to 80 days. Histological images were obtained at several time points to compare with the corresponding OCT images. Volume measurements were also acquired.

Results

OCT can identify the key features observed in corresponding histological images based on the scattering differences from various eye tissues, such as the cornea, intact and regenerated lens, and the iris. Lens volume measurements from three-dimensional OCT images showed that the re

SUBMITTER: Chen W 

PROVIDER: S-EPMC8362625 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature