Human airway mast cells proliferate and acquire distinct inflammation-driven phenotypes during type 2 inflammation.

ABSTRACT: Mast cells (MCs) play a pathobiologic role in type 2 (T2) allergic inflammatory diseases of the airway, including asthma and chronic rhinosinusitis with nasal polyposis (CRSwNP). Distinct MC subsets infiltrate the airway mucosa in T2 disease, including subepithelial MCs expressing the proteases tryptase and chymase (MC_TC) and epithelial MCs expressing tryptase without chymase (MC_T). However, mechanisms underlying MC expansion and the transcriptional programs underlying their heterogeneity are poorly understood. Here, we use flow cytometry and single-cell RNA-sequencing (scRNA-seq) to conduct a comprehensive analysis of human MC hyperplasia in CRSwNP, a T2 cytokine-mediated inflammatory disease. We link discrete cell surface phenotypes to the distinct transcriptomes of

SUBMITTER: Dwyer DF 

PROVIDER: S-EPMC8362933 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature