Quantifying dynamic range in red blood cell energetics: Evidence of progressive energy failure during storage.

ABSTRACT:

Background

During storage, red blood cells (RBCs) undergo significant biochemical and morphologic changes, referred to collectively as the "storage lesion". It was hypothesized that these defects may arise from disrupted oxygen-based regulation of RBC energy metabolism, with resultant depowering of intrinsic antioxidant systems.

Study design and methods

As a function of storage duration, the dynamic range in RBC metabolic response to three models of biochemical oxidant stress (methylene blue, hypoxanthine/xanthine oxidase, and diamide) was assessed, comparing glycolytic flux by NMR and UHPLC-MS methodologies. Blood was processed/stored under standard conditions (AS-1 additive solution) with leukoreduction. Over a 6-week period, RBC metabolic and antioxidant status were asses

SUBMITTER: Rogers SC 

PROVIDER: S-EPMC8363157 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature